Open AccessMolecular monitoring of the transcriptional activation of the yeast Saccharomyces kluyveri mating pheromone signal transduction by using FUS1‐lacZ fusion gene
Author(s) -
Fujimura Hiroaki
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05282.x
Subject(s) - saccharomyces cerevisiae , mating of yeast , yeast , mechanism (biology) , mating type , biology , lac operon , mating , pheromone , gene , signal transduction , biochemistry , microbiology and biotechnology , genetics , gene expression , philosophy , epistemology
To analyse the molecular mechanism by which a mating pheromone signal is transmitted in Saccharomyces kluyveri , the S. cerevisiae FUS1‐lacZ fusion gene was introduced into S. kluyveri cells and its transcriptional activation was investigated. The expression of FUS1‐lacZ fusion product was cell‐type‐specifically induced by pheromone treatment in S. kluyveri . The result suggests that S. kluyveri pheromone signal may be transduced by a mechanism similar to that in S. cerevisiae . This system may provide a model for analysing the molecular mechanism of the mating reaction in Saccharomyces kluyveri .