Open AccessElectrotransformation of the stable L‐form of Proteus mirabilis
Author(s) -
Katenkamp Ulrich,
Groth Ingrid,
Laplace Frank,
Malke Horst
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05281.x
Subject(s) - proteus mirabilis , plasmid , transformation (genetics) , protoplast , electroporation , recombinant dna , polyethylene glycol , microbiology and biotechnology , dna , biology , chemistry , bacteria , genetics , biochemistry , gene , staphylococcus aureus
To improve the transformability of stable protoplast type L‐forms of Proteus mirabilis for recombinant plasmid DNA, conditions for efficient electrotransformation were explored. Exposing cells from the exponential phase of growth at a density of 6−8 × 10 9 /ml in electrotransformation buffer having a conductivity of 1.4 mS/cm to a field strength of 6.5 kV/cm for a mean pulse duration time of 1.2 ms reproducibly yielded transformation efficiencies in the order of 5 × 10 4 transformants per μg of DNA. Compared to the polyethylene glycol method for transformation, electrotransformation appeared to be the method of choice for introduction of plasmid DNA into L‐form cells.