Open AccessAspergillus niger G proteins: subcellular localization
Author(s) -
Mérit Xavier,
FrotCoutaz Jacques,
Got René,
Létoublon Robert
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05271.x
Subject(s) - endoplasmic reticulum , cytosol , aspergillus niger , cell fractionation , subcellular localization , biochemistry , gtp' , membrane , microsome , biology , enzyme , chemistry , cytoplasm
Aspergillus niger postmitochondrial fraction, which contains high GTPase activity and high GTP binding capacity, has been subjected to subcellular fractionation on a sucrose gradient. A cytosolic and four membranous populations have been separated according to their relative density. The main difficulty has been the characterization of the plasma membrane of the fungus. This fraction, which does not contain any typical enzyme, has been identified after iodination of the outer proteins of protoplasts from A. niger . The immunological detection has shown the occurrence of cytosolic G proteins and membranous small G proteins located not only in the plasma membrane but also in the membranes of the endoplasmic reticulum.