Open AccessCharacterization of lipopolysaccharides from four Pasteurella haemolytica serotype strains: evidence for presence of sialic acid in serotypes 1 and 5
Author(s) -
Utley Suzanne R.,
Bhat U.Ramadas,
Byrd Wyatt,
Kadis Solomon
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05263.x
Subject(s) - serotype , myristic acid , microbiology and biotechnology , sialic acid , palmitic acid , stearic acid , pasteurella , bacteria , fatty acid , biology , chemistry , biochemistry , genetics , organic chemistry
Highly purified lipopolysaccharides (LPS) obtained from four strains of Pasteurella haemolytica representative of four different serotypes were studied to ascertain their overall structural elements and sugar and fatty acid compositions. SDS‐PAGE analysis revealed that each LPS was of the smooth‐type although they differed in migration patterns. Somewhat unusual features of these LPS included the presence of: (a) rhammose in the core oligosaccharides of serotypes 2 and 3; and (b) sialic acid in the LPS of serotypes 1 and 5. The fatty acids, myristic, hydroxymyristic and palmitic occur in essentially equivalent amounts in each of these LPS. In addition, stearic acid was present in small amounts in serotypes 1 and 5.