Open AccessNucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus
Author(s) -
JatonOgay Katia,
Suter Mark,
Crameri Reto,
Falchetto Rocco,
Fatih Abdelouahad,
Monod Michel
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05253.x
Subject(s) - aspergillus fumigatus , complementary dna , biology , nucleic acid sequence , peptide sequence , gene , microbiology and biotechnology , genomic dna , cdna library , genomic library , protease , biochemistry , enzyme
An Aspergillus fumigatus extracellular alkaline protease (ALP) which is an enzyme of the subtilisin family is a potential virulent factor of the fungus. The gene encoding ALP was isolated from a genomic library made from DNA of an A. fumigatus isolate. The nucleotide sequence of this gene was compared to that of a cDNA encoding A. oryzae ALP and to that of a cDNA from A. fumigatus encoding the mature ALP protein. Mature A. fumigatus ALP contains 282 amino acids and is encoded by three exons. The pre‐proenzyme has a leader sequence of 121 amino acids.