Open AccessComparison of Vibrio parahaemolyticus hemolysin (Vp‐TRH) produced by environmental and clinical isolates
Author(s) -
Yoh Myonsun,
Miwatani Toshio,
Honda Takeshi
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05252.x
Subject(s) - hemolysin , vibrio parahaemolyticus , microbiology and biotechnology , toxin , antigenicity , virulence , biology , vibrionaceae , bacteria , antibody , biochemistry , gene , immunology , genetics
TDH‐related hemolysin (Vp‐TRH) produced by Kanagawa‐phenomenon‐negative (KP − ) Vibrio parahaemolyticus has been demonstrated to be a possible virulence determinant. Though almost half of KP − isolates examined from diarrhoeal patients produced Vp‐TRH, few reports mentioned the ability of environmental isolates to produce Vp‐TRH. Considering the route of infection with V. parahaemolyticus , this toxin must be produced by the organisms in the sea or in sea food. To confirm that Vp‐TRH produced by V. parahaemolyticus could be involved in sea‐food‐borne diarrhoeas, Vp‐TRH‐producing strains were isolated from the environment, identified and hemolysin purified from these strains was compared to hemolysin (Vp‐TRH) isolated from diarrhoeal patients. The results showed that the hemolytic activity, antigenicity, reactivity in the rabbit ileal loop test and N‐terminal amino acid sequence of Vp‐TRH from environmental strains was indistinguishable from the toxin of clinical origin.