Open AccessCombined influence of magnesium concentration and polymerase chain reaction specificity enhancers
Author(s) -
Riedel K.H.J.,
Wingfield B.D.,
Britz T.J.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05236.x
Subject(s) - enhancer , magnesium , polymerase chain reaction , chemistry , microbiology and biotechnology , polymerase , biochemistry , biology , dna , gene , organic chemistry , gene expression
The importance of an optimal magnesium concentration (6.5 mM), as well as the inclusion of a specific polymerase chain reaction enhancer, to obtain the correct fragment after amplification was examined during this study. In contrast to tetramethylammonium chloride, dimethyl sulphoxide did not enhance the specificity of amplification at different magnesium concentrations, but rather altered the specificity, resulting in the amplification of a larger fragment.