Open AccessThe promoter element GTACAAG of the SGA and STA2 genes is a possible target site for repression by the STA10 gene product from Saccharomyces cerevisiae
Author(s) -
Claros Manuel Gonzalo,
Pozo Lourdes,
Abarca Dolores,
Jiménez Antonio
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05234.x
Subject(s) - saccharomyces cerevisiae , gene , psychological repression , promoter , biology , genetics , transcription (linguistics) , gene product , transposable element , gene expression , genome , linguistics , philosophy
The SGA and STA2 genes that, respectively, encode the intra‐ and extracellular glucoamylases of Saccharomyces cerevisiae are coregulated negatively, at the level of transcription, by the STA10 gene. This finding was re‐examined by determining the effects of STA10 on the expression of gene constructs containing different fragments from the SGA and STA2 promoter regions fused to the lacZ gene. Repression was observed only for promoter fragments carrying the sequence GTACAAG indicating that this element is responsible for the coregulation of SGA and STA2 by STA10 .