Open AccessInitial reactions in the mineralization of 2‐sulfobenzoate by Pseudomonas sp. RW611
Author(s) -
Hansen Corinna,
Fortnagel Peter,
Wittich RolfMichael
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05231.x
Subject(s) - sulfite , chemistry , mineralization (soil science) , pseudomonas , dioxygenase , sulfur , ammonium , sulfate , substituent , enzyme , biodegradation , ammonium sulfate , catechol , nucleophile , nitrogen , biochemistry , stereochemistry , organic chemistry , bacteria , catalysis , biology , genetics
Pseudomonas sp. strain RW611 utilized the ammonium salt of 2‐sulfobenzoate as sole source of carbon, sulfur, nitrogen, and energy. The xenobiotic sulfo substituent was dioxygenolytically eliminated as sulfite, which was then slowly oxidized to sulfate. 2,3‐Dihydroxybenzoate, which resulted from desulfonation underwent meta ‐cleavage, mediated by 2,3‐dihydroxybenzoate 3,4‐dioxygenase activity. This enzyme was inhibited by 3‐chlorocatechol and 2,3,4‐trihydroxybenzoate.