Open AccessHistone HMf from the hyperthermophilic archaeon Methanothermus fervidus binds to DNA in vitro using physiological conditions
Author(s) -
Stroup Diane,
Reeve John N.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05220.x
Subject(s) - puc19 , histone , dna , in vitro , plasmid , chemistry , biochemistry , microbiology and biotechnology , biology
The concentration of HMf (histone Methanothermus fervidus ) in vivo has been shown to be between 1 and 2 × 10 4 molecules per genome. At this mass ratio the amount of HMf bound to pUC19 DNA in vitro was found to be dependent on the topology of the plasmid DNA. M. fervidus grows optimally between 80 and 85°C and contains approx. 1 M K + plus 300 mM 2′3′(cyclic) diphosphoglycerate. (Hensel, R. and König, H. 1988. FEMS Microbiol. Lett. 49, 75–79). HMf binding to DNA in vitro under these conditions has been demonstrated.