Open AccessEucaryotic cell components that bind to chlamydial elementary bodies: the histones
Author(s) -
Vretou Evangelia,
Eliades Petros,
Psarrou Evgenia,
Kouvatsou Raina
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05213.x
Subject(s) - hela , histone , microbiology and biotechnology , biology , chromatin , cell , cell membrane , dna , biochemistry
HeLa‐cell‐membrane fractions isolated by sonication as used previously to identify chlamydial adhesins were examined by a blotting technique for binding chlamydial elementary bodies (EB). One HeLa cell protein with apparent molecular mass of 32 kDa was found to bind native EB. A monoclonal antibody (mAb) raised against this chlamydial binding host‐cell protein reacted with eucaryotic histones. Histone fractions were capable of binding EB in an ELISA assay and histone H1 was identified as the chlamydial‐binding host cell protein in the Hela cell membrane fraction. Probing with specific mAbs against histone H3 and DNA confirmed that chromatin components were present in the host‐cell membrane extract. These data suggest that the HeLa‐cell‐binding chlamydial proteins were previously identified by their reaction with chromatin and not with membrane components.