Open AccessCloning and nucleotide sequence of the gene encoding the γ‐ d ‐glutamyl‐ l ‐diamino acid endopeptidase II of Bacillus sphaericus
Author(s) -
Hourdou MarieLaure,
Duez Colette,
Joris Bernard,
Vacheron MarieJeanne,
Guinand Micheline,
Michel Georges,
Ghuysen JeanMarie
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05203.x
Subject(s) - bacillus sphaericus , endopeptidase , nucleic acid sequence , diamino acid , peptide sequence , biology , biochemistry , signal peptide , gene , cloning (programming) , enterococcus faecium , microbiology and biotechnology , molecular cloning , protein primary structure , enzyme , genetics , bacillales , bacteria , bacillus subtilis , gene sequence , antibiotics , programming language , phylogenetic tree , computer science
The gene encoding the Bacillus sphaericus γ‐ d ‐glutamyl‐ l ‐diamino acid endopeptidase II, a cytoplasmic enzyme involved in cell sporulation [1], contains the information for a 271‐amino acid protein devoid of a signal peptide. The endopeptidase lacks sequence relatedness with other proteins of known primary structure except that its C‐terminal region has significant similarity with the C‐terminal region of the 54‐kDa P54 protein of Enterococcus faecium , of unknown function [2].