Open AccessChanges in double‐stranded RNA profiles in Agaricus bisporus during subculture
Author(s) -
Morten K.J.,
Hicks R.G.T.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05202.x
Subject(s) - basidiocarp , biology , rna silencing , mycelium , subculture (biology) , phenotype , rna , microbiology and biotechnology , genetics , botany , gene , rna interference , taxonomy (biology)
A complex double‐stranded RNA (dsRNA) pattern found in mycelial cultures of diseased basidiocarps comprised nine major segments between 0.78–3.6 kbp and four minor segments between 8.8–15 kbp. With extensive subculturing, a new pattern of five segments between 1.5–8.8 kbp was detected in cultures expressing the diseased phenotype. Loss of the 1.5 and 3.6 kbp segments following heat therapy at 30°C was associated with remission of symptoms. Some of the dsRNAs co‐purified with mitochondria in extracts from diseased cultures and basidiocarps.