Open AccessChlorate resistant mutants of Pseudomonas stutzeri affected in respiratory and assimilatory nitrate utilization and expression of cytochrome cd 1
Author(s) -
Zumft Walter G.,
Blümle Sandor,
Braun Cornelia,
Körner Heinz
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05201.x
Subject(s) - nitrate reductase , nitrogen assimilation , nitrate , pseudomonas stutzeri , mutant , chlorate , nitrite reductase , biochemistry , biology , chemistry , cytochrome , microbiology and biotechnology , enzyme , gene , genetics , inorganic chemistry , bacteria , ecology
Chlorate‐resistant mutants were generated by random insertion of the transposon Tn5 into genomic DNA of Pseudomonas stutzeri ZoBell strain and selected for loss of nitrate respiration (Nar phenotype). The mutants were differentiated by restriction‐fragment analysis, by assaying for nitrate assimilation and for molybdenum co‐factor activity, and by the amount of respiratory nitrate reductase. Two mutants, lacking both nitrate respiration and nitrate assimilation, over‐produced an inactive nitrate reductase but synthesized in the presence of nitrate only a reduced amount of respiratory nitrite reductase (cytochrome cd 1 ). Expression of cytochrome cd 1 in these mutants was specifically induced by nitrate, suggesting a sensor system for this substrate.