Open AccessStructure of the Escherichia coli 0104 polysaccharide and its identity with the capsular K9 polysaccharide
Author(s) -
Kogan Grigorij,
Jann Barbara,
Jann Klaus
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05198.x
Subject(s) - polysaccharide , escherichia coli , chemistry , periodate , galactose , lipopolysaccharide , biochemistry , ultracentrifuge , neuraminic acid , chromatography , microbiology and biotechnology , biology , sialic acid , gene , endocrinology
The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104: K − , a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long‐chain LPS. Compositional analysis, NMR spectroscopy, periodate oxidation and methylation analysis showed that the polysaccharide chain of O104 LPS II consisted of galactose, N‐acetylgalactosamine and neuraminic acid and acetate in the molar ratio of 2: 1: 1: 1 and contained 3‐βGal, 3‐βGalNAc, 4‐αGal, and 4‐α(9‐OAc‐NeuNAc) in linear sequence. The same results were obtained with the capsular K9 polysaccharide from E. coli O9: K9, as presented here and reported previously (Dutton et al. (1987) Carbohydr. Res. 170, 193–206).