Open AccessCell‐free synthesis and membrane‐integration of the reaction center subunit H from Rhodobacter capsulatus
Author(s) -
Troschel Dorothee,
Eckhardt Susan,
Hoffschulte Hedda K.,
Müller Matthias
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05197.x
Subject(s) - rhodobacter , membrane , photosynthetic reaction centre , membrane protein , protein subunit , biochemistry , cell free system , urea , cell free protein synthesis , trypsin , chemistry , protein biosynthesis , biology , enzyme , photosynthesis , gene , mutant
The H‐subunit of the photosynthetic reaction center of Rhodobacter capsulatus was synthesized in vitro by programming a R. capsulatus cell‐free translation system with isolated H‐protein mRNA. When intracytoplasmic membrane vehicles of R. capsulatus were added cotranslationally, newly synthesized H‐protein sedimented on sucrose gradients with the membranes. The majority of the membrane‐associated H‐protein was resistant towards extraction with 6 M urea or 0.2 M Na 2 CO 3 at pH 10.5. The urea‐extractibility, however, increased significantly when membranes had been treated with trypsin prior to the insertion of the H‐subunit, suggesting that the membrane assembly of the H‐protein is dependent on (a) proteinaceous factor(s).