Open AccessClonal differences within M‐types of the group A streptococcus revealed by pulsed field gel electrophoresis
Author(s) -
Single Leigh A.,
Martin Diana R.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05188.x
Subject(s) - restriction fragment length polymorphism , restriction enzyme , serotype , biology , pulsed field gel electrophoresis , gel electrophoresis , restriction fragment , genotype , microbiology and biotechnology , genetics , streptococcaceae , streptococcus , dna , bacteria , gene
Abstract Digestion of chromosomal DNA with the rare cutting restriction enzyme Sfi I in association with pulsed field gel electrophoresis was used to observe restriction fragment length polymorphisms (RFLP) among isolates of group A Streptococcus. Streptococci examined included isolates belonging to the same M‐type (epidemiologically related and unrelated), and isolates from other M‐types. RFLP patterns were quite distinct between all serotypes tested. More importantly, isolates from within a serotype could be differentiated by this technique.