Open AccessCloning of a Clostridium botulinum type B toxin gene fragment encoding the N‐terminus of the heavy chain
Author(s) -
Ho Jung Hyun,
Rhee Sang,
Yang KyuHwan
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05185.x
Subject(s) - clostridium botulinum , cloning (programming) , fragment (logic) , gene , toxin , molecular cloning , microbiology and biotechnology , biology , genetics , peptide sequence , computer science , programming language
Abstract Two λgt11 clones of the toxin gene of Clostridium botulinum type B were identified by the monoclonal antibody specific to the heavy chain of type B toxin. Neither of the expressed fusion protein from the lysates of lysogenic E. coli Y1089 showed any botulinal toxic activity. One of the clones hybridized to the oligonucleotide probe which was synthesized according to the amino acid sequence of N‐terminus of heavy chain. The sequence analysis revealed that highly homologous regions in N‐terminus of heavy chain exist among botulinum neurotoxins (type A, B) and tetanus toxin on the amino acid sequence level.