Open AccessReplication of staphylococcal plasmid pT48
Author(s) -
Catchpole I.R.,
Dyke K.G.H.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05183.x
Subject(s) - plasmid , gene , biology , replication (statistics) , dna replication , origin of replication , genetics , linker , dna , rolling circle replication , microbiology and biotechnology , virology , computer science , operating system
Insertion of a synthetic DNA linker into the repL gene of staphylococcal plasmid pT48 inactivates the replication system. This defect can be complemented in trans by the presence of a pT48 repL gene, but not by the rep genes of the related Staphylococcus areus plasmids pSN2 and pOX1000. Comparison of the sequences of the three replication proteins indicates that specificity may be determined by a putative helixturn‐helix region.