Open AccessCharacterization of the genes coding for the F 1 F 0 subunits of the sodium dependent ATPase of Propionigenium modestum
Author(s) -
Krumholz Lee R.,
Esser Ursula,
Simoni Robert D.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05180.x
Subject(s) - gene , biology , homology (biology) , coding region , escherichia coli , plasmid , atpase , amino acid , genetics , biochemistry , microbiology and biotechnology , enzyme
The DNA coding for the eight structural genes and unc I of the sodium dependent ATPase of Propionigenium modestum has been cloned and sequenced. Based on sequence homology, the genes were determined to appear in the order uncBEFHAGDC as in several other bacterial species. Minicell experiments revealed that plasmids containing the P. modestum DNA expressed those ATPase polypeptides in Escherichia coli . These were very similar in molecular mass to those obtained from the purified ATPase of P. modestum . No membrane‐bound ATPase activity was observed in E. coli unc deletion strains containing the P. modestum ATPase genes. Amino acid alignments which were done with the Fo subunits revealed only a few conservative changes in the highly conserved regions of the polypeptides.