Open AccessMechanism of action of a cytotonic enterotoxin produced by Aeromonas hydrophila
Author(s) -
Chopra Ashok K.,
Vo Tuyet N.,
Houston Clifford W.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05177.x
Subject(s) - aerolysin , enterotoxin , aeromonas hydrophila , microbiology and biotechnology , biology , cholera toxin , chinese hamster ovary cell , toxin , monoclonal antibody , bacteria , antibody , biochemistry , escherichia coli , immunology , gene , receptor , genetics
Abstract In this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of Aeromonas hydrophila . Isolates SSU and Ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. A cytotonic enterotoxin produced by diarrheal isolate SSU, which was purified and characterized in our laboratory, elevated intracellular cAMP and PgE 2 levels in cultured Chinese hamster ovary (CHO) cells. Likewise, enterotoxic activity expressed by a cytotonic enterotoxin was detected in the culture filtrate of a fish isolate (Ah65) after cytotoxic activity was neutralized with homologous aerolysin monoclonal antibodies. This cytotonic enterotoxin also elevated intracellular cAMP and PgE 2 levels in CHO cells, suggesting a cholera toxin‐like mechanism of action for Aeromonas cytotonic enterotoxins.