Open AccessA structurally novel staphylococcal protein A from the V8 strain
Author(s) -
FinckBarbançon V.,
Prevost G.,
Mazurier I.,
Piemont Y.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05175.x
Subject(s) - strain (injury) , escherichia coli , amino acid , leucine , staphylococcus aureus , methionine , peptide sequence , gene , biology , microbiology and biotechnology , chemistry , biochemistry , bacteria , genetics , anatomy
Protein A from the Staphylococcus aureus strain V8 has a molecular mass about 8000 Da less than that of known proteins A. The corresponding gene was cloned and expressed in Escherichia coli . Sequence analysis of this structurally new protein A revealed that it lacked an IgG‐binding domain (58 amino acids), and that it also lacked two octapeptide repetitions located in the membrane/wall attaching region. Contrary to what had been proposed previously, the first translated amino acid is probably not a leucine, but very likely a methionine located 12 residues upstream.