Open AccessPhysical mapping of the mitochondrial DNA from Aspergillus niger
Author(s) -
Kirimura Kohtaro,
Fukuda Satoshi,
Abe Hidetoshi,
Kanayama Shinji,
Usami Shoji
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05158.x
Subject(s) - mitochondrial dna , restriction enzyme , restriction map , aspergillus niger , dna , biology , microbiology and biotechnology , restriction fragment , genetics , biochemistry , gene , plasmid
The mitochondrial DNA was isolated from Aspergillus niger WU‐2223L, a citric acid‐production strain, and characterized by restriction‐endonuclease mapping. Cloned fragments which covered the total range of the mitochondrial DNA were assembled and utilized to construct the restriction‐endonuclease map for nine restriction enzymes. This map showed that the mitochondrial DNA was a circular molecule of 32.6 kb.