Open AccessInhibition of respiratory oxidation of elemental sulfur (S 0 ) and thiosulfate in Thiobacillus versutus and another sulfur‐oxidizing bacterium
Author(s) -
Beffa Trello,
Berczy Margaret,
Aragno Michel
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05138.x
Subject(s) - thiosulfate , chemistry , sulfite , respiratory chain , sulfur , cytochrome , thiobacillus , cytochrome c , ferricyanide , inorganic chemistry , medicinal chemistry , photochemistry , nuclear chemistry , biochemistry , organic chemistry , enzyme , mitochondrion
The rates of thiosulfate, elemental sulfur (S 0 ) and sulfite oxidation were measured respirometrically with an oxygen electrode using young cells of Thiobacillus versutus growing chemolithoautotrophically on thiosulfate under normal air pressure. Myxothiazol, an inhibitor of the cytochrome b−c 1 segment, and HQNO (2‐N‐heptyl‐4‐hydroxyquiniline N‐oxide), acting in the quinone‐cytochrome b region, both significantly inhibited the thiosulfate oxidation rate. The effect on the oxidation rate of S 0 was even stronger. The oxidation of sulfite or ascorbate + TMPD (N,N,N′,N′‐tetramethyl‐p‐phenylenediamine) (substrates releasing electrons at the level of cytochrome c) was not inhibited by myxothiazol and HQNO. Thiosulfate, S 0 , sulfite and ascorbate + TMPD oxidations were strongly inhibited by KCN. These respiratory activities were almost completely eliminated by cell breakage. The reduction of b‐type cytochrome was observed in thiosulfate‐reduced minus sulfite‐reduced difference spectra. This study confirms that S 0 is an important intermediate of thiosulfate oxidation in Thiobacillus versutus , and that electrons released by S 0 oxidation enter the respiratory chain in the quinone‐cytochrome b region. This would allow an increased gain of energy, while less energy would probably be required for pyridine‐nucleotide reduction.