Open AccessCharacterization of Serpulina (Treponema) hyodysenteriae and related intestinal spirochetes by ribosomal RNA gene restriction patterns
Author(s) -
Jensen Neil S.,
Casey Thomas A.,
Stanton Thad B.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05103.x
Subject(s) - treponema , biology , restriction enzyme , ribosomal rna , gene , 16s ribosomal rna , microbiology and biotechnology , restriction map , restriction fragment , ribosomal dna , genetics , virology , plasmid , phylogenetics , syphilis , human immunodeficiency virus (hiv)
Serpulina (Treponema) hydysenteriae strain A‐1 partially purified rRNA, labelled with photobiotin, was used as a non‐radioactive probe to identify the rRNA gene restriction patterns of S. hyodysenteriae strains and other spirochetes. Sau 3A restriction enzyme digests resulted in similar rRNA gene restriction patterns in S. hyodysenteriae strains from five different countries. Some S. hyodysenteriae strains could be differentiated by variations in their rRNA gene restriction patterns after cleavage of DNA by restriction enzymes Ssp I or Bgl II. S. innocens and Treponema succinifaciens , non‐pathogenic pig intestinal spirochetes, had rRNA gene restriction patterns that differed markedly from the S. hyodysenteriae patterns, and from each other.