Open AccessLipid accumulation in Trichoderma species
Author(s) -
SerranoCarreon Leobardo,
Hathout Yetrib,
Bensoussan Maurice,
Belin JeanMarc
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05087.x
Subject(s) - trichoderma harzianum , trichoderma viride , biomass (ecology) , dry weight , food science , sucrose , sodium nitrate , chemistry , botany , ammonium nitrate , microorganism , ammonium , biology , biochemistry , agronomy , bacteria , organic chemistry , biological pest control , genetics
Two filamentous fungi, Trichoderma harzianum and Trichoderma viride , were compared for their ability to synthesize lipids on different carbon and nitrogen sources. Three culture media were selected for each strain after preliminary screening. All the test media were nitrogen‐deficient (C/N = 60) so as to stimulate lipid accumulation. For both microorganisms the glucose‐ammonium sulphate medium was the most conducive to lipid production: a lipid accumulation of 17% (w/w) of biomass dry weight was obtained for T. harzianum and of 32% (w/w) of biomass dry weight for T. viride . In sucrose‐sodium nitrate medium T. harzianum was able to accumulate almost 25% (w/w) of its biomass in lipid form. However the small quantity of biomass produced (2 g dry weight/l) limited the quantity of lipid obtained. Neutral lipids, free fatty acids and phospholipids were monitored during 8 days of cultivation of the two fungi.