Open AccessBordetella heat‐labile toxin causes release of radioactivity from smooth muscle cells labeled with [ 14 C]arachidonic acid
Author(s) -
Nagai Masaaki,
Endoh Masahiko,
Burns Drusilla L.,
Nakase Yasukiyo
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05079.x
Subject(s) - arachidonic acid , toxin , chemistry , phosphatidylcholine , biochemistry , microbiology and biotechnology , biology , phospholipid , enzyme , membrane
The effect of Bordetella heat‐labile toxin (HLT) on cultured vascular or tracheal smooth cells labeled with [ 14 C]arachidonic acid for 3 h was examined. At 37°C, in the presence of Ca 2+ , HLT induced the release of radioactive substances from the cells in a dose‐dependent manner but HLT had no effect on release of radioactivity from cells at 0°C or in the absence of Ca 2+ . After cells were exposed to HLT, a 2‐h lag period occurred before release of radioactivity was detected. The substances released from cells by HLT were identified as arachidonic acid and phosphatidylcholine.