Open AccessNucleotide sequence of the methoxyneurosporene dehydrogenase gene * from Rhodobacter sphaeroides : Comparison with other bacterial carotenoid dehydrogenases
Author(s) -
Garí Eloi,
Toledo Joan C.,
Gibert Isidre,
Barbé Jordi
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05048.x
Subject(s) - rhodobacter , rhodobacter sphaeroides , biology , peptide sequence , gene , nucleic acid sequence , homology (biology) , rhodospirillaceae , sequence analysis , biochemistry , sequence alignment , genetics , microbiology and biotechnology , mutant , photosynthesis
The nucleotide sequence of the 1794‐bp fragment containing the crtD gene from Rhodobacter sphaeroides 2.4.1 encoding for methoxyneurosporene dehydrogenase has been determined. A 63% sequence identity was found when compared with the nucleotide sequence of the crtD gene from Rhodobacter capsulatus . A putative regulatory palindromic motif present in the crtD gene from R. capsulatus also exists in this gene from R. sphaeroides . The translated open reading frame of the crtD gene of R. sphaeroides has identified a polypeptide of 495 amino acids which shares a 56% sequence identity with the same CrtD protein of R. capsulatus . The N‐ and C‐ termini of these CrtD proteins present a high degree of similarity with the N‐ and C‐termini of other carotenoid dehydrogenases including those encoded by crtI genes. This is in good agreement with the previously hypothesized homology between CrtI and CrtD proteins.