Open AccessMurine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization
Author(s) -
Goyache Joaquín,
Orden JoséA.,
Blanco JoséL.,
Hernández Javier,
Doménech Ana,
Suárez Guillermo,
GómezLucía Esperanza
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05002.x
Subject(s) - enterotoxin , monoclonal antibody , antibody , microbiology and biotechnology , chemistry , serotype , spleen , biology , immunology , escherichia coli , biochemistry , gene
A group of 14 monoclonal antibodies (mAbs) to staphylococcal enterotoxin B (SEB) were obtained by fusion of Sp2/O myeloma cells with spleen cells from female BALB/c mice immunized with commercial SEB. The antibodies belonged to IgG1 and IgG2b subclasses. We evaluated the anti-SEB titres, competition assays and sensitivity of detection by indirect ELISA. Reactivity and cross-reactivity were also studied by indirect ELISA and confirmed by immunoblotting. All the mAbs reacted with SEB and with a second band which had a different electrophoretic mobility and probably represents an aggregate of SEB or SEB bound to membranes. Three mAbs reacted only with SEB and the rest showed cross-reactions with SEC1. No reactions were observed against any other serovar (SEA, SED and SEE) or other proteins.