Open AccessPolycrylamide gel electrophoresis analysis of lipopolysaccharide from Pseudomonas aeruginosa growing planktonically and as biofilm
Author(s) -
Giwercman Birgit,
Fomsgaard Anders,
Mansa Bendt,
Høiby Niels
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb04998.x
Subject(s) - pseudomonas aeruginosa , biofilm , lipopolysaccharide , microbiology and biotechnology , bacteria , cystic fibrosis , gel electrophoresis , pseudomonadaceae , pulsed field gel electrophoresis , polyacrylamide gel electrophoresis , biology , chemistry , immunology , biochemistry , gene , enzyme , genetics , genotype
Lipopolysaccharide (LPS, endotoxin) was extracted from biofilm and planktonically grown monoagglutinable (1118) and polyagglutinable (258 and 15703) strains of Pseudomonas aeruginosa isolated from cystic fibrosis patients with chronic pulmonary infections. Analysis by polyacrylamide gel electrophoresis (PAGE) followed by immune‐detection of LPS fractions showed an S‐form appearance of strain 1118 and 258 with three distinct clusters of high molecular weight bands, whereas 15703 appeared semi‐rough. LPS of semi‐rough cells grown planktonically and as biofilm showed a very similar PAGE pattern; however, the core/lipid A R‐LPS fraction was more prominent in biofilm‐LPS than in planktonic‐LPS extracted from the S‐form bacteria (1118 and 258). The apparent change in LPS sub‐unit components of the bacteria when grown as biofilm may reflect changes in the outer membrane structure that contribute to the altered physico‐chemical properties of biofilm bacteria in foreign‐device associated infections and chronic P. aeruginosa lung infection in cystic fibrosis patients.