Open AccessImmunochemical characterization of Citrobacter strain PCM 1487 O‐specific polysaccharide‐ and core oligossaccharide‐protein conjugates
Author(s) -
Ługowski Czesław,
Kułakowska Małgorzata,
Romanowska Elż;bieta
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb04995.x
Subject(s) - shigella flexneri , antiserum , toxoid , polysaccharide , chemistry , oligosaccharide , shigella sonnei , microbiology and biotechnology , citrobacter , shigella , epitope , antigen , lipopolysaccharide , biochemistry , enterobacteriaceae , biology , escherichia coli , immunization , genetics , gene , endocrinology
O-specific-polysaccharide and core oligosaccharide were isolated from Citrobacter strain PCM 1487 lipopolysaccharide and purified. The polysaccharide was selectively devoid of 4-deoxy-D-arabinohexose residues. Covalent conjugates of the modified O-specific polysaccharide and of the core oligosaccharide with tetanus toxoid were prepared. Immunochemical characterization of these conjugates proved that they are strong immunogens. Using monospecific rabbit antisera raised against the conjugates, the antigenic relationships between lipopolysaccharides of various strains of Citrobacter, Shigella sonnei, and Shigella flexneri were studied by quantitative microprecipitin and quantitative microprecipitin inhibition and immunoblotting tests.