Open AccessAntibody response to Staphylococcus aureus whole cell, lipase and staphylolysin in patients with S. aureus infections
Author(s) -
Ryding U.,
Renneberg J.,
Rollof J.,
Christensson B.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb04976.x
Subject(s) - staphylococcus aureus , serology , endocarditis , lipase , antibody , medicine , microbiology and biotechnology , micrococcaceae , triacylglycerol lipase , immunology , enzyme , biology , bacteria , biochemistry , genetics
Three assays to measure antibodies against Staphylococcus aureus whole cells, lipase and staphylolysin were used to try to discriminate between complicated and uncomplicated S. aureus septicaemia. Sera were examined from 8 patients with S. aureus endocarditis, 23 patients with complicated S. aureus septicaemia, 12 patients with uncomplicated S. aureus septicaemia and 93 febrile non‐septicaemic controls. No single assay could distinguish between complicated and uncomplicated S. aureus septicaemia. If the criterion for a positive result is defined as positive antibody level in the anti‐lipase ELISA as well as in at least 1 of the other 2 assays, 10/31 patients with S. aureus endocarditis or complicated septicaemia were positive compared to 0/93 non‐septicaemic patients and 0/12 patients with uncomplicated S. aureus septicaemia. Therefore, the combined use of serological assays in the diagnosis of complicated S. aureus septicaemia, one of which is the anti‐lipase ELISA, is recommended.