Open AccessRNA polymerase heterogeneity in Streptomyces aureofaciens : characterization by antibody‐linked polymerase assay
Author(s) -
Farkašovský Marian,
Kormanec Ján,
Kollárová Marta
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb05125.x
Subject(s) - streptomyces aureofaciens , rna polymerase , polymerase , microbiology and biotechnology , biology , rna polymerase i , rna dependent rna polymerase , rna polymerase ii , sigma factor , escherichia coli , streptomyces , dna , gene , promoter , biochemistry , gene expression , genetics , bacteria
Using antibody‐linked polymerase assay we studied the polypeptide composition of DNA‐dependent RNA polymerase from Streptomyces aureofaciens and immunological cross‐reaction with Escherichia coli RNA polymerase. We identified about 25 ‘ALPA‐reactive’ polypeptides which are probably involved in the transcriptional apparatus. We demonstrated that β′ and β subunits from S. aureofaciens and E. coli are immunologically related and σ 70 (E. coli) shows immunochemical similarity with σ 35 (S. aureofaciens). According to the reconstitution of RNA polymerase holoenzyme and antibody‐linked polymerase assay we identified sigma factors responsible for recognition of two promoters.