Open AccessMolecular cloning of the ViaB region of Salmonella typhi
Author(s) -
Hashimoto Yasuhiro,
Ezaki Takayuki,
Li Na,
Yamamoto Hiroyuki
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb05124.x
Subject(s) - salmonella typhi , citrobacter freundii , southern blot , plasmid , biology , escherichia coli , microbiology and biotechnology , cloning (programming) , molecular cloning , dna , cosmid , restriction map , enterobacteriaceae , gene , genetics , peptide sequence , computer science , programming language
The ViaB region required for Vi antigen production in Salmonella typhi was cloned. The plasmid pGBM124 containing a 14‐kb S. thypi chromosomal DNA fragment conferred the ability to produce Vi antigen on Escherichia coli HB101 and ViaB ‐deleted S. typhi GIFU10007‐3. Tn5 insertion analysis showed that the 14‐kb DNA was split into three regions. Region 1 and region 2 are involved in the biosynthesis of Vi polysaccharide. Region 3 is involved in translocation of the Vi polysaccharide to the cell surface. Southern blot hybridization showed that regions 2 and 3 but not region 1, were considerably homologous to the DNA of Vi‐positive Citrobacter freundii .