Open AccessCytofluorometric analysis of anti‐lymphocyte antibodies in AIDS
Author(s) -
Bonner B.C.,
Poulton T.A.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04967.x
Subject(s) - phytohaemagglutinin , lymphocyte , antibody , cd8 , immune system , spleen , immunology , microbiology and biotechnology , chemistry , t lymphocyte , biology
Anti‐lymphocyte antibodies (ALA) have been detected in the plasma of 53.8% of HIV‐positive patients tested (CD4/CD8 ratios: mean 0.265; range 0.01 to 0.5) using analytical continuous‐flow cytofluorometry. IgG from the AIDS plasma was seen to bind to normal PBL in 53.8% of cases (14/26). In double labelling experiments CD4 + lymphocytes, CD8 + lymphocytes, and B lymphocytes were all bound by the ALA, but monocytes were not bound. Pre‐adsorption of the diluted AIDS plasma onto an excess of mouse spleen cells did not remove lymphocyte binding activity. No evidence was found for preferential binding to phytohaemagglutinin‐stimulated lymphocytes. ALA could not be detected in the plasma of normal subjects, patients with acute renal failure undergoing renal dialysis, or patients with high levels of circulating immune complexes.