Open AccessA chloramphenicol‐streptomycin‐resistance plasmid from a clinical strain of Staphylococcus sciuri and its structural relationships to other staphylococcal resistance plasmids
Author(s) -
Schwarz Stefan,
GrölzKrug Sabine
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04902.x
Subject(s) - plasmid , biology , microbiology and biotechnology , staphylococcus aureus , streptomycin , restriction enzyme , southern blot , chloramphenicol , genetics , gene , antibiotics , bacteria
A 5.1‐kb plasmid, designated pSCS12, isolated from a naturally occurring Staphylococcus sciuri conferred resistance to chloramphenicol (Cm R ) and streptomycin (Sm R ). Restriction endonuclease analyses of pSCS12 revealed partial structural homologies to the Cm R ‐plasmids pC221 from S. aureus and pSCS1 from S. intermedius , to the Sm R ‐plasmids pSAI‐1 from S. hyicus and pS194 from S. aureus , as well as to the Cm R /Sm R plasmid pSK68 from S. aureus . Southern‐blot hybridization with specific Cm R ‐ and Sm R ‐gene probes confirmed these similarities and allowed the mapping of the Cm R ‐ and Sm R ‐determinants in the S. sciuri plasmid pSCS12. These observations lead to the suggestion that Cm R /Sm R ‐plasmids, such as pSCS12, may have evolved from Cm R ‐ and Sm R ‐plasmids by interplasmidic recombination.