Open AccessEthanol metabolism in a peroxisome‐deficient mutant of the yeast Hansenula polymorpha
Author(s) -
Sulter G.J.,
Klei I.J.,
Schanstra J.P.,
Harder W.,
Veenhuis M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04898.x
Subject(s) - isocitrate lyase , microbody , biochemistry , malate synthase , peroxisome , mutant , glyoxylate cycle , chemostat , yeast , biology , isocitrate dehydrogenase , metabolism , ethanol metabolism , alcohol dehydrogenase , cytosol , wild type , ethanol , enzyme , malate dehydrogenase , gene , genetics , bacteria
This paper describes ethanol metabolism in a peroxisome‐deficient (PER) mutant of Hansenula polymorpha . The PER mutant was able to use ethanol as sole‐carbon source but showed reduced growth rates compared to wild‐type cells together with a reduced rate of ethanol utilization under μ max conditions. In chemostat cultures at low‐dilution rates, the activities of alcohol dehydrogenase, isocitrate lyase and malate synthase were comparable in wild‐type and PER cells. In PER cells the two latter enzymes, exclusively microbody‐bound in wild‐type cells, were active in the cytosol. The possible advantage of intact microbodies in the intermediary metabolism of ethanol in H. polymorpha is discussed.