Open AccessIsolation of nuclei from Aspergillus nidulans protoplasts
Author(s) -
Vágvölgyi Csaba,
Ferenczy Lajos
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04889.x
Subject(s) - aspergillus nidulans , protoplast , mycelium , biology , density gradient , centrifugation , aspergillus , nuclear dna , fungus , filamentous fungus , chemistry , biochemistry , chromatography , microbiology and biotechnology , botany , enzyme , gene , physics , quantum mechanics , mutant , mitochondrial dna
Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans . The large amounts of protoplasta required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density‐gradient centrifugation was applied. The resulting nuclei were of good purity and morphological state, as demonstrated by fluorescence microscopy and electronmicroscopy. The weight ratio of DNA: RNA: protein was 1: 3.0: 10.8 in the nuclear fraction.