Open AccessOccurrence of pap‐, sfa‐ , and afa ‐related sequences among F165‐positive Escherichia coli from diseased animals
Author(s) -
Harel Josée,
Daigle France,
Maiti Sourindra,
Désautels Clarisse,
Labigne Agnès,
Fairbrother John M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04861.x
Subject(s) - operon , bacterial adhesin , escherichia coli , biology , microbiology and biotechnology , homology (biology) , genetics , gene
Abstract: A total of 160 Escherichia coli positive for F165 fimbrial antigen and isolated from diarrheic and septicemic animals, were examined for the presence of the pap, afa , and sfa/foc operons or related nucleotide sequences using colony hybridization. Most isolates shared DNA sequences with the pap operon sequences alone or in association with afa or sfa . Thus, our results indicate that F165‐positive E. coli from diseased animals share DNA sequences with operons coding for adhesins important in human extra‐intestinal disease and that multiple adhesin systems are often found in single isolates. However, 20% of the F165‐positive isolates did no show any homology with the probes representing the three adhesin systems, suggesting that one of the operons responsible for F165 production could be different from the pap, sfa/foc , and afa operons.