Open AccessNatural abundance 13 C‐nuclear magnetic resonance spectroscopic analysis of acyclic polyol and trehalose accumulation by several yeast species in response to salt stress
Author(s) -
Meikle Amanda J.,
Chudek John A.,
Reed Robert H.,
Gadd Geoffrey M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04859.x
Subject(s) - debaryomyces hansenii , ribitol , trehalose , polyol , glycerol , osmoregulation , arabitol , yeast , osmotic shock , osmotic pressure , biochemistry , nuclear magnetic resonance spectroscopy , chemistry , biology , xylitol , salinity , enzyme , stereochemistry , fermentation , organic chemistry , ecology , gene , polyurethane
Analysis of seventeen yeast strains by 13 C‐NMR spectroscopy has confirmed the significance of glycerol as the sole osmoregulatory solute under salt‐stressed conditions, and has shown arabitol to be present in most of the osmotolerant species. Ribitol was detected in some species, including Debaryomyces hansenii , although ribitol accumulation did not correlate with the osmotic pressure of the medium. Relative amounts of arabitol and ribitol decreased in relation to glycerol when the external osmotic pressure was increased. Trehalose was present during exponential growth of some species.