Open AccessTransformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae ‐based plasmids
Author(s) -
Fujimura Hiroaki
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04856.x
Subject(s) - ura3 , saccharomyces cerevisiae , plasmid , transformation (genetics) , yeast , saccharomyces , mutant , biology , genetics , biochemistry , gene , chemistry
For the transformation of the yeast Saccharomyces kluyveri, ura 3 mutants were obtained by 5‐fluoro‐orotic acid selection. By utilizing the method based on treatment of intact cells with alkali cations, the ura 3 strains of S. kluyveri were transformed by Saccharomyces cerevisiae ‐based plasmids. In the transformed cells, a S. cerevisiae centromere‐based plasmid was stably replicated autonomously. Thus, this system will permit the study of gene expression and its regulation in S. kluyveri in relationship to that in S. cerevisiae .