Open AccessCloning of regulator genes controlling fimbrial production by enterotoxigenic Escherichia coli
Author(s) -
Willshaw Geraldine A.,
Smith Henry R.,
McConnell Moyra M.,
Rowe Bernard
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04852.x
Subject(s) - escherichia coli , cloning (programming) , fimbria , regulator , gene , biology , microbiology and biotechnology , regulator gene , enterobacteriaceae , gene expression , genetics , computer science , programming language
Sequences regulating production of fimbriae were cloned from two enterotoxigenic Escherichia coli strains. One cloned region, from E. coli 025.H42, controlled expression of coli surface‐associated (CS) antigen 4, whereas the function of the other, from E. coli 0167.H5, was unclear. Both regulators were related to the cfaD gene that controls expression of colonization factor antigen I (CFA/I) although low stringency conditions were required to show significant hybridization between cfaD and the regulatory fragment from E. coli 0167. The cloned regulatory genes promoted expression of CFA/I, CS1, CS2 and CS4 antigens but the levels of production in the presence of the 0167 regulator were lower than those promoted by the CS4 regulator of cfaD .