Open AccessEvolutionary lines among Salmonella enteritidis phage types are identified by insertion sequence IS 200 distribution
Author(s) -
Stanley John,
Jones Clive S.,
John Threlfall E.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04844.x
Subject(s) - biology , genetics , phage typing , salmonella enteritidis , salmonella , insertion sequence , genome , lineage (genetic) , southern blot , typing , dna , gene , bacteria , transposable element
A survey was made of the presence, copy number and location of the Salmonella ‐specific DNA insertion element IS 200 , within the genomes of the 27 phage type strains of Salmonella enteritidis . All the phage type strains contained copies of IS 200 revealed by genomic Southern blot hybridizations with a 300‐bp DNA probe internal to the element. Restriction site variation around IS 200 insertion sites was examined. Three fundamental patterns of hybridization corresponding to chromosomal IS 200 loci were found. In terms of population genetics, these ‘IS 200 profiles’ correspond to clonal lineages of recent evolutionary origin, and underlike the phage‐typing scheme for epidemiological subdivision of S. enteritidis . The molecular analysis is consistent with genetic selection pressures which are apparent in the observed epidemiological distribution of S. enteritidis , since each clonal lineage contained one of the phage types of major clinical importance in the U.K.