Open AccessFusion of mycoplasmas: the formation of cell hybrids
Author(s) -
Tarshis Mark,
Salman Michael,
Rottem Shlomo
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04841.x
Subject(s) - cell fusion , hybrid , fusion , biology , computational biology , microbiology and biotechnology , cell , genetics , botany , philosophy , linguistics
Poly(ethylene glycol) (PEG 8000) can induce cell‐cell fusion of Mycoplasma capricolum cells, and it can promote the formation of intergeneric hybrids of various Mycoplasma, Acholeplasma and Spiroplasma species. The extent of fusion was quantitatively evaluated by following the dequenching of octadecylrhodamine fluorescent label incorporated into donor cell membranes after their incubation with recepient cells. The results of dequenching experiments were confirmed by electron microscopy, as well as by angle light‐scattering measurements. Fusion appeared to require the presence of Mg 2+ , but was completely inhibited by either 0.1% glutaraldehyde or 100 μM chlorpromazine, and was partially suppressed by proteolytic enzymes, carbonyl cyanide‐ m ‐chlorophenylhydrazone, or thiol reagents.