Open AccessThymidine incorporation into lake water bacterioplankton and pure cultures of chemolithotrophic (CO, H 2 ) and methanotrophic bacteria
Author(s) -
Kraffzik Brunhilde,
Conrad Ralf
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04790.x
Subject(s) - bacterioplankton , bacteria , thymidine , paracoccus denitrificans , biology , pseudomonas , heterotroph , microbiology and biotechnology , bacterial growth , biochemistry , environmental chemistry , chemistry , ecology , dna , phytoplankton , nutrient , genetics
Incorporation of [ 3 H]methyl thymidine into bacterial DNA was measured using samples of bacterioplankton from Lake Constance and pure cultures of CO, H 2 and CH 4 ‐oxidizing bacteria. Thymidine was incorporated by Pseudomonas carboxydovorans, Paracoccus denitrificans, Methylosinus trichosporium, Methylomonas agile , and by various chemolithotropic or methylotrophic isolates from Lake Constance. Thymidine incorporation by bacterial cultures was stimulated by increasing concentrations of CO or H 2 . Increased CH 4 concentrations stimulated thymidine incorporation by Ms. trichosporium only if the cells had been starved. In contrast to bacterial cultures, thymidine incorporation by bacterioplankton samples was not stimulated by increasing