Open AccessLack of specific hybridization between the lep genes of Salmonella typhimurium and Bacillus licheniformis
Author(s) -
Dijl Jan Maarten,
Jong Anne,
Smith Hilde,
Bron Sierd,
Venema Gerard
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04784.x
Subject(s) - bacillus licheniformis , gene , salmonella , biology , signal peptidase , clone (java method) , microbiology and biotechnology , dna , genetics , bacteria , peptide sequence , signal peptide , bacillus subtilis
This paper describes an attempt to clone the Bacillus licheniformis lep gene, encoding signal peptidase, using the Salmonella typhimurium lep gene as a hybridization probe. Although a hybridizing fragment was obtained, DNA sequence analysis indicated that it did not contain the lep gene. Instead, the protein encoded by the cloned fragment showed similarity with a variety of l ‐asparaginases.