Open AccessDetection and characterization of naturally occurring plasmids in Bacillus licheniformis
Author(s) -
Parini C.,
Fortina M.G.,
Manachini P.L.,
Rossi E.,
Riccardi G.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04781.x
Subject(s) - bacillus licheniformis , plasmid , bacillus subtilis , biology , homology (biology) , bacillaceae , bacillales , microbiology and biotechnology , dna , hindiii , genetics , gene , bacteria
Twenty‐two Bacillus licheniformis strains, freshly isolated from pasture‐land, were studied for the presence of plasmid DNA. Among these strains, 14 were shown to harbor one or more plasmids of different size. Southern‐hybridization experiments showed a high homology between all plasmids investigated and a 2.2‐kb Pvu II/ Hin dIII fragment of pBL1, a B. licheniformis plasmid previously isolated. Three fragments of pBL1, including the 2.2‐kb Pvu II/ Hin dIII region, were cloned into pJH101 vector. The resulting chimeras were able to transform Bacillus subtilis . The fragment with high homology probably contains the region with the replicative functions of plasmids from B. licheniformis species.