Open AccessThe respiratory response to heat shock in Neurospora crassa
Author(s) -
Habel Donald,
PlesofskyVif Nora,
Bramblk Robert
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04779.x
Subject(s) - neurospora crassa , cytoplasm , glycogen , respiration , mitochondrion , fluorescence microscope , biology , ultrastructure , biophysics , electron microscope , shock (circulatory) , oxygen , biochemistry , chemistry , fluorescence , anatomy , medicine , physics , mutant , optics , organic chemistry , quantum mechanics , gene
A sharp decrease in oxygen uptake occurred in Neurospora crassa cells that were transferred from 30°C to 45°C, and the respiration that resumed later at 45°C was cyanide‐insensitive. Energization of mitochondria, measured in vivo with fluorescence microscopy and a carbocyanine dye, also declined sharply in cells at 45°C. Electron microscopy showed no changes in mitochondrial complexity; however, the cytoplasm of heat‐shocked cells was deficient in glycogen granules.