Open AccessProduction of monoclonal antibody to Clostridium difficile toxin A which neutralises enterotoxicity but not haemagglutination activity
Author(s) -
Kamiya S.,
Yamakawa K.,
Meng X.Q.,
Ogura H.,
Nakamura S.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04778.x
Subject(s) - monoclonal antibody , toxin , isotype , clostridium difficile toxin a , microbiology and biotechnology , clostridium difficile , hemagglutination , biology , antibody , chemistry , virology , immunology , antibiotics
Nine monoclonal antibodies (mAb) to Clostridium difficile toxin A were produced. The isotype of one mAb (37B5) was IgG2b, kappa, and that of the other eight mAbs was IgM, kappa. Immunoblot analysis after non‐denatured PAGE showed that with the exception of one mAb (112G6) all mAbs gave a positive reaction with the 540 kDa band of toxin A. Immunoblot analysis showed that four mAbs (2E15, 3B4, 37B5 and 49C4) gave a positive reaction with the 240 kDa major band of toxin A. In neutralisation tests with these mAbs for enterotoxicity, mouse lethality, haemagglutination activity and cytotoxicity, 37B5 neutralised enterotoxicity in a rabbit ileal loop response test but did not neutralise any other biological activities. None of the other eight mAbs showed any neutralising activities at all.