Open AccessAnalysis of the human serological response to Chlamydia trachomatis 60‐kDa proteins by two‐dimensional electrophoresis and immunoblotting
Author(s) -
Coles Anthony M.,
Crosby Heather A.,
Pearce John H.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04776.x
Subject(s) - antiserum , biology , chlamydia trachomatis , isoelectric focusing , serology , microbiology and biotechnology , gel electrophoresis , chlamydiales , chlamydiaceae , immunodiffusion , isoelectric point , antibody , virology , immunology , biochemistry , enzyme
Earlier analyses of sera from humans and animals with chlamydial infections demonstrated a strong immune response to proteins of approximately 60 kDa. We have used two‐dimensional (2D) electrophoresis followed by immunoblotting to more accurately define patient responses to the chlamydial 60‐kDa cysteine‐rich envelope protein (OMP2) and to the 57‐kDa stress protein (SP) implicated in immunopathological damage. OMP2 and SP were separated on 2D gels by their distinct isoelectric points and identified by fluorography of [ 35 S]‐labelled proteins and cross‐reaction with anti‐mycobacterial antiserum, respectively. The majority of patients sera showed a strong reaction both to OMP2 and the SP (18/20 and 17/20 sera, respectively). Fewer sera (9/20 and 10/20 sera, respectively) reacted with two other polypeptides also present in the 60‐kDa range by this analysis.